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RamAp enhances <t>superoxide</t> <t>dismutase</t> <t>(SOD)</t> activity in Salmonella LT2. SOD activity was measured in crude lysates of S. enterica serovar Typhimurium LT2 carrying either pBR322 or pBR322-IS Ecp1* - ramAp using a commercial assay kit. Activity values were normalized to total protein concentration. Each data point represents an independent biological replicate ( n = 3); horizontal lines indicate the mean and standard deviation. A significant increase in SOD activity was observed in the ramAp -expressing strain.
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RamAp enhances superoxide dismutase (SOD) activity in Salmonella LT2. SOD activity was measured in crude lysates of S. enterica serovar Typhimurium LT2 carrying either pBR322 or pBR322-IS Ecp1* - ramAp using a commercial assay kit. Activity values were normalized to total protein concentration. Each data point represents an independent biological replicate ( n = 3); horizontal lines indicate the mean and standard deviation. A significant increase in SOD activity was observed in the ramAp -expressing strain.

Journal: Frontiers in Microbiology

Article Title: Plasmid-borne transcriptional regulator RamAp modulates Salmonella genes for environmental and host adaptation

doi: 10.3389/fmicb.2026.1842592

Figure Lengend Snippet: RamAp enhances superoxide dismutase (SOD) activity in Salmonella LT2. SOD activity was measured in crude lysates of S. enterica serovar Typhimurium LT2 carrying either pBR322 or pBR322-IS Ecp1* - ramAp using a commercial assay kit. Activity values were normalized to total protein concentration. Each data point represents an independent biological replicate ( n = 3); horizontal lines indicate the mean and standard deviation. A significant increase in SOD activity was observed in the ramAp -expressing strain.

Article Snippet: Crude lysates were prepared and analyzed using the CheKineTM Micro Superoxide dismutase (SOD) Activity Assay Kit (Abbkine, Atlanta, GA, USA), following the manufacturer’s instructions.

Techniques: Activity Assay, Protein Concentration, Standard Deviation, Expressing